Yeast-2G-UNic®
Vector Technology
Premium expression vectors for fungal and yeast protein production platforms®
Seamlessly integrate Yeast-2G UNic® into your workflow to unlock advancements in protein yield (up to ten-fold improvements).
Embrace innovation as we redefine cell factory development, offering high-yield performance and cost-effective solutions tailored for yeast platforms.
Discover Yeast-2G UNic® vector technology
Yeast-2G UNic® vector technology dramatically improves multiple aspects of the protein expression pathway. It drives an increase in protein yields in fungal and yeast protein production platforms.
The technology significantly boosts transcription, mRNA stability, transport as well as enhancing translation to drive an increase in protein expression.
Impact and advantages of Yeast-2G UNic® on protein production
Production: severalfold higher protein production levels
Scalability: ensures stable or enhanced yields in fed batch bioreactor runs.
Reproducibility: demonstrates increased production in over 90% of tested proteins.
Promoter agnostic: achieves over a twofold yield increase with constitutive and inducible promoters.
Compatibility: enables multifold titer enhancement in Pichia and other yeast strains.
Broad applicability: extends beyond biopharmaceuticals to industrial enzymes, food, and feed sectors.
Validated: by external parties, confirming its reliability and versatility.
Strong IP position: Fully owned IP in the US, Europe and other regions with independent patent families.
How does Yeast-2G UNic® vector technology work?
Yeast-2G UNic® integrates innovative yeast-specific genetic components, collectively enhancing recombinant protein expression within yeast cells.
Drawing parallels to ProteoNic’s expression vectors for mammalian platforms, Yeast-2G UNic® augments transcription and translation rates, yielding:
More mRNA per integrated DNA copy
Experience a significant boost in transcription in your yeast strain. Benefit from increased transcription levels achieved through:- Dual Promoter: Harness the patented technology of two promoters ingeniously merged to amplify transcription.
More protein per mRNA molecule
Enhance protein production through significant boosts in mRNA stabilty, transport and translation.- Improved translation efficiency by optimized ribosome affinity
- Optimized mRNA transport by use of a synthetic intron
- Enhanced mRNA stability through increased ribosome binding and longer mRNA half-life
Case study:
effect of Yeast-2G UNic® vector technology on the production of a biopharmaceutical protein in yeast
(Pichia pastoris/Komagataella phaffii)
- Strain selection: identified optimal strains for recombinant protein expression.
- Copy-number amplification: amplified gene copy numbers for enhanced expression.
- Comparison: evaluated the performance of the best reference strain against the top Yeast-2G UNic® strain
- Yield increase: achieved a remarkable 6-fold increase in protein yield in a 30L bioreactor
- Conclusion: demonstrated the significant efficacy of Yeast-2G UNic® in enhancing protein expression in yeast, promising for industrial applications.
Other applications of 2G UNic® vector technology
Protein production in mammalian cell lines
Increase the expression of recombinant proteins
in all CHO-based platforms
Lentivirus titer improvement in mammalian cell lines
Increase viral vector titers for boosting cell and gene therapy manufacturing capacity
Boost AAV transgene expression
Increase AAV transgene/payload expression for your gene therapy to boost therapeutic efficacy
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