Recombinant protein production in cultured cells
In recombinant protein production, cells are reprogrammed to produce specific proteins by insertion of a new gene into the cell genome. Following this process, the gene is transcribed into RNA (transcription), which in turn is used as a template for protein production (translation). The newly synthesized protein molecules are transported through the cell and – in most cases – secreted into the culture medium from which it can be isolated. The various steps in the process impact recombinant protein production levels, and need to be optimized for efficient, high level production.
2G UNic™ Translation Enhancing Elements (TEE) increase recombinant protein production
Translation enhancement: not just more mRNA
UNic™ enhances production efficiency by increasing recombinant protein output per cell. It specifically boosts translation, not transcription while leaving production methods, including cell types and culture methods, completely unchanged. UNic™ can be combined with technologies which increase gene expression at other levels, such as transcription, transport and secretion.
ProteoNic’s 2G UNic™ technology for improving recombinant protein production is protected by patents issued in the US and Europe. The patents cover all eukaryotic cell types used in recombinant protein production (including mammalian cells, yeast and fungal cells), as well as the TEE sequences and their use. All rights of patents and patent applications reside within ProteoNic.