Premium vector technology driving improved productivity and quality
Protein production in
mammalian cell lines
Increase the expression of recombinant proteins in all mammalian platforms
Viral vector performance in mammalian cell lines
Boost viral vector titers or payload expression in mammalian platforms
Protein production in
yeast and fungal strains
Increase the expression of recombinant proteins in yeast and fungal platforms
Increase the expression of your recombinant proteins in mammalian platforms
2G UNic®: premium vector technology for increased protein production levels
A combination of proprietary genetic elements in one vector, together driving higher protein production levels
More mRNA per integrated DNA copy
Increased transcription levels due to:
- Double promoter: two promoters are combined in a novel way to increase transcription
- Epigenetic stabilization: reduced epigenetic down-regulation allows stable high-level expression
More protein per mRNA molecule
- Improved translation efficiency by optimized ribosome affinity
- Optimized mRNA transport by use of a synthetic intron
- Optimized mRNA stability
- Superior performance: multi-fold increase in recombinant protein productivity over the entire range of expression levels:
- Low level productivity (< 1 g/l) to commercially viable levels
- Medium to high levels, e.g., from 2 to 5 g/l
- High end levels, e.g., from 5 to 8 g/l
- Broad applicability:
- Across all relevant mammalian cell lines and expression systems
- For different types of molecules
- For random and targeted integration; multi- and single copy
- Easy process integration: upgrade current processes with minimal changes to systems, workflow or product quality
- Reduce screening effort: fewer resources required to identify high-producing clones
- Compatible and synergistic: combine with other expression-enhancing technologies to further boost productivity, e.g., transposon or chromatin modulation technology
- Significant savings: potentially save millions of euros per year, per product (based on typical volumes)
- Further growth opportunities: can be applied to other in-demand areas, e.g., viral vector and vaccine production, mRNA therapeutics, production in yeast and other fungal cells, etc.
- Strong IP position: Fully owned IP with four independent patent families in the US and Europe
Suitable for a broad range of proteins, cell types, and selection markers, including:
Interested in trying 2G UNic™ for your application?
Read more about the product formats and licenses here
- Easy and efficient vector cloning: one-step construction
- Compatible with GoldenGate assembly
- Mono- and bicistronic vector formats available
- Straightforward preparation of transfection-ready linear DNA by Shredder technology (patent pending)
Conclusion: Superior titer obtained using 2G UNic™
Protein: Bispecific antibody
Cell line: CHO GS-/- (HD-BIOP3)
Approach: 2G UNic™ vector
Results: 3-fold increased titers with 2G UNic™ vectors
Webinar
Unleashing the full potential of CHO cells: What is the importance of vector technology?
Listen to our insightful webinar where we delve into strategies for unlocking the full potential of CHO cells using cutting-edge vector technologies in cell line development.
- Optimizing gene expression
- Achieving scalable yield and enhanced quality
- Navigating Production Challenges
Boost viral vector titers or payload expression in mammalian platforms
2G UNic®: premium vector technology for boosting viral vector performance in mammalian cell lines
A combination of proprietary genetic elements driving increased viral vector titers and/or payload expression
- Works across all relevant mammalian cell lines and expression systems
- Compatible with Lentivirus (LV) or Adeno-associated virus (AAV) viral vector delivery platforms
- Boost titer or payload expression
- Transient or stable expression
- Inducible or constitutive expression
- Superior performance: significant increase of lentiviral particle titer levels:
- 2G UNic technology applied to multiple 3rd Generation LV vectors for increased LV titers
- Virus produced in HEK LV-MAX suspension cultures in shake flasks
- Performed with CDMO partner (NecstGen)
- Exceptional performance:
- 2G UNic vector technology boosts expression of a gene of interest (GOI) in human cells transduced with AAV
- Significantly increased expression when fused to a constitutive promoter (CMV)
- Boost expression from tissue-specific promoters
- 2G UNic vector technology boosts expression of a gene of interest (GOI) in human cells transduced with AAV
- Significant manufacturing savings: potentially save millions of euros per year, per product (based on typical volumes)
- Boost manufacturing capacity manufacturing savings: increase your viral vector titers by two-four fold and reduce your current cell growth batch sizes to achieve same titers.
- Easy process integration: upgrade current processes with minimal changes to systems, workflow or product quality
- Compatible and synergistic: combine with other expression-enhancing technologies to further boost productivity
More mRNA per integrated DNA copy (stable expression)
Increased transcription levels due to:
- Dual promoter system: two promoters are combined in a novel way to increase transcription
- Epigenetic stabilization: reduced epigenetic down-regulation allows stable high-level expression
More protein per mRNA molecule (stable & transient expression)
- Improved translation efficiency by optimized ribosome affinity
- Optimized mRNA transport by use of a synthetic intron
- Optimized mRNA stability by increased ribosome binding
- 2G UNic technology track record: adopted by Top 20 Pharma to boost protein production in CHO cell line systems
- Clinically validated: therapies using 2G UNic incorporated in their manufacturing platform are in phase II studies and beyond
- Strong IP position: Fully owned IP with four independent patent families in the US and Europe
Interested in joining our Early Access Program?
Improve expression levels in yeast platforms
2G-P UNic™: premium vector technology for increased protein production levels in yeast
Premium expression vectors for fungal and yeast protein production platforms
2G-P UNic™ technology has been optimized and validated for reliable application
- Severalfold higher higher protein production levels
- Scalable: yields stable, or further improved, in fed batch bioreactor runs
- Reproducible: production increased in >90% of proteins tested
- Independent of promoter type: >2-fold yield increase achieved with constitutive and inducible promoters
- Pichia compatible: multifold titer enhancement achieved in Pichia
- Broadly applicable: applications beyond biopharmaceuticals include industrial enzymes, food, feed
- Validated by external parties
2G-P UNic™ is a combination of novel yeast-specific genetic elements which together improve recombinant protein expression in yeast cells
In analogy to ProteoNic's expression vectors for mammalian platforms, 2G-P UNic™ increases transcription and translation rates, resulting in:
- More mRNA per integrated DNA copy
- More protein per mRNA molecule
Are you interested in trying 2G-P UNic™ for your application?
Effect of 2G-P UNic™ on production of a biopharmaceutical protein in yeast (Pichia pastoris)
Approach: Strain selection with copy-number amplification; comparison of best reference strain with best 2G-P UNic™ strain
Results: 6-fold yield increase in 30 L bioreactor
Interested in trying 2G-P UNic™ for your application?
ST6 sialylation technology: a glyco-engineering approach for improved protein quality
Achieve human-type sialylation of biotherapeutic proteins in CHO cells for improved efficacy and reduced immunogenicity
- High yield & superior protein quality combined
- Easy process integration: apply by simple co-transfection
- CHO cell line compatible, including GS and DHFR knockout lines
- Replaces human cell platforms: sialylation levels similar to serum IgGs and IVIg under standard culture conditions
- Versatile sialyl transferase vector:
- Combined with 2G UNic™ vectors provides high biotherapeutic titers with superior product quality in any CHO system
- Licensed separately for transfection into an existing cell line
- Analysis and optimization services and consultancy available
A chimeric ST6 minigene in an expression vector with selectable marker
- ST6 activity is absent in wild-type CHO cells and expressed proteins are poorly sialylated
- One-step approach for human-type 6-sialylation by stable expression of novel modified ST6
- Efficient human-type 6-sialylation of N-glycans in the Fc region of IgGs and Fc-fusion proteins in CHO cells
Conclusion: Human serum-like sialylation of mAb achieved by co-transfection with novel ST6 Protein: IgG1 Cell line: CHOZN; bulk pools Approach: Co-transfection of 2G UNic™ vectors for mAb and ST6 Results:
- Increased sialylation level to ≈15% with ST6, similar to intravenous immunoglobulins (IVIg)
- ST6 has a minimal impact on IgG1 expression levels
- Sialylation levels >75% can be obtained by clone selection and feed optimization while maintaining high expression levels
Interested in trying ST6 for your application?
We are continuously developing new applications for our protein-boosting technology
Additional applications of ProteoNic's technology
2G UNic® is applicable to a wide range of other applications:
- Transient protein expression
- Baculovirus-based production in insect cells
- mRNA-based therapeutic development
- Vaccine production
- Various non-human or non-medical applications