Find the right product to boost your protein production
Increase your protein yield and quality in a wide range of mammalian expression platforms
Improve the expression of your recombinant proteins in yeasts and fungi
Available product:
Note: All vectors and elements are available under license and are supplied with expert support and protocols to ensure optimal technology transfer.
2G UNic™: a premium vector technology for increased protein production in mammalian cell lines
We offer our 2G UNic™ vectors in two formats, depending on your requirements: both formats are suitable for mono- or bicistronic vector designs.
2G UNic™ vectors and elements have been extensively validated in all commonly used platforms and are available for licensing.
Option 1:
Complete 2G UNic™ vector
Insert your gene of interest into the 2G UNic™ vector
- Developed and optimized for different cell types and selectable marker systems, including:
- GS
- DHFR
- Antibiotic markers (including blasticidin, zeocin, puromycin, etc.)
- Easy and efficient vector cloning: one-step construction of single gene and bicistronic expression vectors
- Compatible with GoldenGate assembly
- Easy removal of bacterial genes from vector backbone
Vector variants that are not readily available can be generated on request.
Option 2:
2G UNic™ elements
Add 2G UNic™ promoter/enhancer modules to improve your existing vector
- Boost performance while maintaining the desired features of your existing vector
- Compatible with any vector system and technology on the market, even state-of-the-art vectors and expression enhancing genetic elements, including:
- S/MARs
- Transposons
- Chromatin modulators
Our experience with a wide variety of other elements and technologies means we can quickly provide expert advice on the best way to apply 2G UNic™ elements in an existing vector.
ST6: a glyco-engineering approach to improve protein quality in mammalian cell lines
Our novel one-step glyco-engineering technology increases the human-type sialylation of biotherapeutic proteins.
- Suitable for any CHO system, including GS and DHFR KO cell lines
- Applications include
- Production of IVIgs
- Therapeutic antibodies for auto-immune indications
- Available in two formats
Option 1:
Insert your gene of interest into the 2G UNic™ vector and co-transfect with the ST6 vector into your cell line to combine high biotherapeutic titers with human-type sialylation
Option 2:
Transfect your existing production cell line with ST6 vector to obtain human-type sialylation of the biotherapeutic product
We also provide consultancy and services for glycosylation analytics and optimization through media.
2G-P UNic™: Improve the expression of your recombinant proteins in yeasts and fungi
Boost protein expression in fungal platforms with our patented and validated 2G-P UNic™ technology
Applications include:
- Biopharmaceuticals
- Industrial enzymes
- Food
- Feed
License 2G-P UNic™ to access:
- Support on vector design for integrating 2G-P UNic™ genetic elements in a platform vector for a functional fusion with the current promoter and selection technology
- Evaluation of 2G-P UNic™ vectors in a yeast, Pichia, or other fungal platform