CHO-2G UNic® Vector Technology
Increase the expression of your recombinant proteins in CHO-based platforms
Explore the transformative CHO-2G UNic® vector technology
Impact of CHO-2G UNic® on protein production
Superior performance: Multi-fold increase in recombinant protein productivity over the entire range of expression levels.
Broad applicability: Across all relevant mammalian cell lines and expression systems for different types of molecules.
Integration: compatible with both random and targeted integration; multi- and single copy.
Easy process integration: upgrade current processes with minimal changes to systems, workflow or product quality.
Reduce screening effort: fewer resources required to identify high-producing clones.
Compatible and synergistic: combine with other expression-enhancing technologies to further boost productivity, e.g., transposon or chromatin modulation technology.
Significant savings: potentially save millions of euros per year, per product (based on typical volumes).
Strong IP position: Fully owned IP with four independent patent families in the US and Europe
| Cell line productivity | Standard vector | CHO-2G UNic® |
|---|---|---|
| Low | <1 g/l | 1 g/l to 3 g/l |
| Medium | 2 g/l to 5g/l | 5 g/l to 8 g/l |
| High | 5 g/l to 8 g/l | 8 g/l to >10 g/L |
Note: Expression depends on target protein and can be greater than the expected values indicated above.
| Cell types | Selection markers | Proteins |
|---|---|---|
| CHO-S | Antibiotic | Antibodies |
| CHO-k1 | DHFR | Bispecific antibodies |
| CHO-DG44 | GS | Fc-fusion proteins |
| CHO-GS-/- (CHOZN, HD-BIOP3) | Large, complex (>300kDa) | |
| HEK | Biosimilars | |
| Difficult to express protein |
Unlock the Power of CHO-2G UNic® Vector Technology
More mRNA per integrated DNA copy
Experience a significant boost in transcription in your CHO cell line. Benefit from increased transcription levels achieved through a unique blend of methodologies:- Dual Promoter: Harness the patented technology of two promoters ingeniously merged to amplify transcription.
- Epigenetic Stabilization: Mitigate epigenetic down-regulation, ensuring sustained high-level expression for optimal results.
More protein per mRNA molecule
Enhance protein production through significant boosts in mRNA stabilty, transport and translation.- Improved translation efficiency by optimized ribosome affinity
- Optimized mRNA transport by use of a synthetic intron
- Enhanced mRNA stability through increased ribosome binding and longer mRNA half-life
Easy to adopt CHO-2G UNic® vector technology
Simple vector construction
- 1-step vector cloning (GoldenGate)
- Single gene or bicistronic
- Available for multiple selection markers
- Available for insertion in client
vector
Efficient stable pool generation
- No protocol changes needed
- High % of high-expressing cells
- Pool data predictive for clone performance (quality & quantity)
Generate superior clones
- Increased titers
- Reducing screening requirements
- Stable expression
- Quality not affected by titer
- Scalable performance
Webinar
Unleashing the full potential of CHO cells: What is the importance of vector technology? Listen to our insightful webinar where we delve into strategies for unlocking the full potential of CHO cells using cutting-edge vector technologies in cell line development.- Optimizing gene expression
- Achieving scalable yield and enhanced quality
- Navigating Production Challenges
Case study:
bispecific antibody production using CHO-2G UNic® vector technology
- Superior performance of 2G-UNic® vector technology over standard 3rd party vector
- Applying 2G UNic® vector technology delivers 33 of top-36 clones producing over 1 g/L in standard fed-batch process with no process development
- Best clones achieve almost 5 g/l and with further process development over 10 g/l (data not shown)
- Competitor vector; only 1 of top-36 clones produces over 1 g/L (~2 g/L)
Other applications of 2G UNic® vector technology
Protein production in
yeast and fungal strains
Increase the expression of recombinant proteins in yeast and fungal platforms
Lentivirus titer improvement
in mammalian cell lines
Increase viral vector titers for boosting cell and gene therapy manufacturing capacity
Boost AAV
transgene expression
Increase AAV transgene/payload expression for your gene therapy to boost therapeutic efficacy
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