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2G UNic® Vector Technology

2G UNic®: a premium vector technology for increased expression in mammalian cell lines and yeast

Our 2G UNic® vector technology provides versatile solutions tailored to your needs, offered in two adaptable formats suitable for mono- or bicistronic vector designs. Backed by comprehensive validation across commonly used platforms, our vector technology and elements are ready for licensing, ensuring seamless integration into your platform.

  • Versatile Formats: Choose from flexible formats based on your specific requirements for mono- or bicistronic vector designs.
  • Extensive Validation: Rigorously tested across commonly used platforms to ensure reliability and compatibility.
  • Licensing Availability: Available for licensing, facilitating easy integration into your projects.
  • Seamless Integration: Designed for effortless incorporation into your existing workflows and experimental setups.
  • Trusted Solution: Benefit from our proven track record and expertise in providing reliable genetic elements for diverse applications.

Adopting 2G UNic® vector technology

Option 1:
Complete 2G UNic® vector

Insert your gene of interest into the 2G UNic® vector

Option 1 is a good choice for CHO-2G UNic® and Yeast-2G UNic®  when you would like to explore a new vector platform
  • Developed and optimized for different cell types and selectable marker systems, including:
    • GS-/-
    • DHFR
    • Antibiotic markers (including blasticidin, zeocin, puromycin, etc.)
  • Easy and efficient vector cloning: one-step construction of single gene and bicistronic expression vectors
  • Compatible with GoldenGate assembly
  • Easy removal of bacterial genes from vector backbone
Vector variants that are not readily available can be generated on request.

Option 2:
2G UNic® elements

Add 2G UNic®;  promoter/enhancer modules to improve your existing vector

Option 2 is a good choice when you would like to maintain your existing vector for CHO-2G UNic® or Yeast-2G UNic®.
  • Boost performance while maintaining the desired features of your existing vector
  • Compatible with any vector system and technology on the market, even state-of-the-art vectors and expression enhancing genetic elements, including:
    • S/MARs
    • Transposons
    • Chromatin modulators

Our experience with a wide variety of other elements and technologies means we can quickly provide expert advice on the best way to apply 2G UNic® elements in an existing vector.

Note: This is the only option for adopting LV-2G UNic® or AAV-2G UNic®

How does 2G UNic® vector technology work?

Unravel the intricacies of 2G UNic® vector technology and its transformative impact on protein titers, viral vector titers and gene expression. Experience a substantial increase in transcription, achieved through a blend of innovative methodologies. Leveraging a dual promoter system, this technology merges two promoters to amplify transcription, while epigenetic stabilization mechanisms mitigate down-regulation, ensuring sustained high-level expression. Moreover, enjoy, enhanced protein production through improvements in mRNA stability, transport, and translation efficiency. This is facilitated by optimized ribosome affinity, synthetic intron usage for mRNA transport, and stability optimization. With 2G UNic® vector technology, elevate your cell line titer, viral vector titer or gene expression performance, unlocking higher yields without compromising quality or cell line characteristics.

More mRNA per integrated DNA copy

Experience a significant boost in transcription. Benefit from increased transcription levels achieved through a unique blend of methodologies:
  • Dual Promoter: Harness the patented technology of two promoters ingeniously merged to amplify transcription.
  • Epigenetic Stabilization: Mitigate epigenetic down-regulation, ensuring sustained high-level expression for optimal results.

More protein per mRNA molecule

Enhance protein production through significant boosts in mRNA stabilty, transport and translation.
  • Improved translation efficiency by optimized ribosome affinity
  • Optimized mRNA transport by use of a synthetic intron
  • Enhanced mRNA stability through increased ribosome binding and longer mRNA half-life
Boost your cell line titer performance with CHO-2G UNic® or Yeast-2G UNic® vector technology, unlock higher yields while maintaining quality and cell line characteristics. Enhance viral vector titers with LV-2G UNic® and boost gene expression with AAV-2G UNic®.

Applications of 2G UNic® vector technology

CHO-2G UNic® for improved protein yields

Increase your protein yield and quality in a wide range of mammalian expression platforms

Yeast-2G UNic® for improved protein yields

Improve the expression of
your recombinant proteins in yeasts and fungi

LV-2G UNic® for improved lentiviral particle titer

Increase your viral vector titers in mammalian expression platforms

AAV-2G UNic® for improved transgene expression

Improve the expression of your transgene / payload for your gene therapy

Note: All vectors and elements are available under license and are supplied with expert support and protocols to ensure optimal technology transfer.

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